CRISPR/Cas9 and Mouse ES Cell Training Class

The University of Michigan Transgenic Animal Model Core is offering a CRISPR/Cas9 and Mouse ES Cell Training Class beginning Monday, April 15, through Friday, April 26.

In this two-week hands-on session, trainees will design, build, and validate CRISPR/Cas9 reagents in mouse embryonic stem (ES) cells. 

Trainees will learn how to work with mouse embryonic stem cells in culture, clone CRISPR/Cas9 reagents in plasmids, and purify and analyze DNA from cultured cells.

Trainees will learn fundamental genetic principles for genetic engineering including:

• Planning CRISPR/Cas9 genome editing workflows,
• Fundamental principles of gene targeting design, and
• How to apply Cre recombinase technology to conditional genes (“floxed genes”).  

In addition, trainees will be introduced to the power of genetically engineered mice to model human disease and learn how to access resources from the International Knockout Mouse Consortium project to knockout every protein coding gene in the mouse.

After attending, trainees will be able to design experiments to create genetic modifications and produce them in mouse ES cells and in mice with CRISPR/Cas9 reagents. Download a sample syllabus (available via PDF download in Dropbox).

How to Register

To reserve a place in this two-week class, log in to MiCORES:

• Navigate to the Transgenic Core

• Click on the Request Services tab

• Click on the Training Classes folder

• Submit a request for CRISPR/Cas9 ES Cell Training Class

Questions?

For questions about this class or U-M's Transgenic Animal Model Core, please email tgcore@umich.edu.